A01-KB104 Application of Structured Light Illumination and Compressed Sensing to High Speed Laminar Optical Fluorescence Tomography

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  • Primary Investigator
    Ichiro Sakuma (The University of Tokyo, School of Engineering, Professor)
  • Co-Researcher
    Masatoshi Yamazaki (The University of Tokyo, School of Engineering,Project researcher (Equivalent to Assistant Professor))
    Haruo Honjo (Nagoya University, Institute of Environmental Medicine, Associate Professor)
    Etsuko Kobayashi(The University of Tokyo, School of Engineering, Associate Professor)
    Keiichi Nakagawa (The University of Tokyo, School of Engineering, Assistant Professor)


Fluorescence image measurement is a useful method as an imaging method. Use of various molecular probes enables measurement of intracellular ion dynamics, membrane potential, localization of specific protein expression in cells, and so on. One of the technical challenges in fluorescence measurement is obtaining information from deep tissue layer from surface. Fluorescence information obtained from the vicinity of the irradiation point of the excitation light source contains information on a relatively shallow tissue portion, whereas fluorescence information obtained from the distal end of the irradiation site is deep tissue portion. Diffusion fluorescence tomography is a method to solve it as an inverse problem of light propagation inside the organization by making use of this principle. However, conventional diffusion fluorescence tomography has limitation in data acquisition speed since it uses raster scanning of excitation light. In this study, we aim to develop a high-speed technique combining compression sensing technology to diffusion fluorescence tomography.

Project Design


Laminar optical fluorescence tomography utilizing structured excitation light illumination and compressive sensing